E.coli ori compability

Dr. Duncan Clark Duncan at nospam.demon.co.uk
Mon Feb 21 11:00:34 EST 2000


In article <88r0j9$ovf$1 at nnrp1.deja.com>, eforsen at my-deja.com writes
>Does anyone have any views on wich oris are compatible and work well together? 
>The copy number should be approx. the same for both plasmids. 

There are several that are compatible, i.e. p15a and colE1 from pACYC177
(or pACYC184) and pBR322 respectively. Your problem is going to be
getting different replicons with similar copy numbers.

Others around include whatever is in pSC101 (1-2 copy no.), anything
with R6K or a modified higher copy R6K (mutated Pi-protein increasing
copy no 4-5fold).

Duncan 
-- 
The problem with being on the cutting edge is that you occasionally get 
sliced from time to time....

Duncan Clark
DNAmp Ltd.
Tel: +44(0)1252376288
FAX: +44(0)8701640382
http://www.dnamp.com
http://www.genesys.demon.co.uk




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