centrifugating colloidal gold

MIguel Moreno mmm42608 at alu.uah.es
Mon Feb 21 12:10:42 EST 2000

Is the first time I'm preparing avidin-gold complexes. I'm having
problems to remove the excess of nonadsorbed protein due to gold
particles don't precipited correctly. I'm centrifugating nore than two
hours at 50000 g. So my cuestion is: Has anybody ever prepared this kind
of complexes.
Thanks of all

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