karlster at excite.com
Mon Feb 21 21:29:35 EST 2000
In article <20000221045925.64332.qmail at hotmail.com>, mimiliao at hotmail.com
("mimi Liao") wrote:
> Dear All...
> The bacterial plasmids i transfected into mammalian cells are able to
> replicate in mammalian cells. However i have trouble distinguishing between
> the bacterial plasmids and the plasmids that have been replicated in the
> mammalian cells. Does anyone know a good protocol for replication assay ?
> Thanks in advance
> Please reply to mimiliao at hotmail.com
You might wish to try Dpn I digestion to distinguish input plasmid from
those which have replicated within target cells; input plasmid will be
digested while replicated should remain untouched.
Sorry I don't have a reference handy but a quick scan of medline or pubmed
should yield something.
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