dialysis device

[Cornelius Krasel]

"Wolfgang Schechinger" <Wolfgang.Schechinger at med.uni-tuebingen.de> wrote:
> I'd like to dialyze a protein purified from E. coli. 
> I'm aware of of a product called slide-a-lyzer from pierce and I'd 
> like to know if this device is useful or if there is something 
> else that might be better. Or should I use conventional dialysis 
> tubing (though I'm afraid that the handling will cause relatively 
> high losses)

I don't know how much protein you have, but usually normal dialysis
tubing (with some plastic clamps, it's easier than to tie a knot
to close the tubing) works fine, and the losses are quite tolerable
(less than 100 ul, I'd guess).

--Cornelius.

-- 
/* Cornelius Krasel, U Wuerzburg, Dept. of Pharmacology, Versbacher Str. 9 */
/* D-97078 Wuerzburg, Germany   email: phak004 at rzbox.uni-wuerzburg.de  SP4 */
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