Annealing Oligos

Andy Scotter pcxajs at unix.ccc.nottingham.ac.uk
Tue Feb 22 11:36:37 EST 2000


	I've got a palindromic 31 base oligo that I want to anneal with
itself so I can blunt clone it into pT7Blue-2.
	Can anyone suggest reasonable conditions and amounts to carry out
the annealing?  I guess it shouldn't be too hard as the oligos should
stick like glue.  After the annealing step I'll be purifying the product
before the end conversion step and ligation.

Cheers,

Andy

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Andy Scotter
Department of Chemistry
University of Nottingham
University Park
Nottingham
NG7 2RD
0115 9514193
pcxajs at unix.ccc.nottingham.ac.uk

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