Enzymatic vitelline layer degradation?

Richard J. Dudley rdudley at pitt.edu
Tue Feb 22 12:37:37 EST 2000


This might help-

Chang & Weiss:
Channel opening locks agonist onto the GABAC receptor.
     Nat Neurosci. 1999 Mar;2(3):219-25.
     PMID: 10195213; UI: 99211117.

Kasper Dreyer wrote:

> Hello Group
>
> I order to perform binding studies with Xenopus oocytes I need to remove the
> vitelline layer. My binding protein is 30 Kd and thus too large to pass the
> vitelline pores but what is the best method to remove the vitelline layer? To my
> knowledge collagenase treatment does not significantly degrade the vitelline
> layer - only the epithelial layer. Obviously you can remove it mechanically but
> is there a better way, e.g. an enzymatic digestion protocol. Is there such a
> thing as "vitellinase"?
>
> This may be a rather elementary question but I'd very much appreciate any help
> on the issue
>
> Kasper Dreyer
> University of Aarhus
> Denmark

--
Richard J. Dudley (rdudley+ at pitt.edu)
Research Specialist V
Cystic Fibrosis Research Center
Dept. of Cell Biology and Physiology
University of Pittsburgh
http://www.cbp.pitt.edu
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