Reprobing Dig Northerns
Mike Challen
mike.challen at hri.ac.uk_nospam
Wed Feb 23 04:25:24 EST 2000
We have been trying to strip and reprobe Northerns (total RNA) , probed
with DIG-DNA. Following recommended strip protocols (Boehringer man.)
we have had no success on second probing, either with the same or
different probes. Does anybody have experience of stripping/reprobing
such Northerns, please? Any fancy protocols for stripping etc?
many thanks
Mike
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