Reprobing Dig Northerns

Mike Challen mike.challen at hri.ac.uk_nospam
Wed Feb 23 04:25:24 EST 2000


We have been trying to strip and reprobe Northerns (total RNA) , probed
with DIG-DNA.  Following recommended strip protocols (Boehringer man.)
we have had no success on second probing, either with the same or
different probes.  Does anybody have experience of stripping/reprobing
such Northerns, please?  Any fancy protocols for stripping etc?

many thanks

Mike





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