T4 RNA Ligase

[Marieluise Kirchner]

used anyone the T4 RNA ligase for ssDNA / oligonucleotide-ligation? in
the buffer of my protocoll hexamine cobalt chloride is used. Why? Is the
effect really that big? Has hexamine cobalt chloride any bad effects in
further reactions like PCR or ligation into a vector?
The question is - should i use this hexamine cobalt chloride or better
not?

thanks and a nice evening

marie

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