T4 RNA Ligase

Marieluise Kirchner kirchner at mpiib-berlin.mpg.de
Wed Feb 23 12:57:31 EST 2000

used anyone the T4 RNA ligase for ssDNA / oligonucleotide-ligation? in
the buffer of my protocoll hexamine cobalt chloride is used. Why? Is the
effect really that big? Has hexamine cobalt chloride any bad effects in
further reactions like PCR or ligation into a vector?
The question is - should i use this hexamine cobalt chloride or better

thanks and a nice evening



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