Band distorsion and RFLP

Errol E.S.Kwan at massey.ac.nz
Wed Feb 23 16:35:27 EST 2000


Have you looked at the electrophoresis and the gel?

On Tue, 15 Feb 2000 15:31:12 -0800, Margarita Mauro
<mmauroherrera at ucdavis.edu> wrote:

>Hi!
>    I'm having problems with my DNA samples in RFLP gels. When I run the
>gels, some of the samples seem to run at a different rate than others.
>The blots hybridize fine, but the bands are so wavy that it's hard to
>tell which are polymorphic or not.
>    I'm isolating genomic DNA from both wild and cultivated accessions
>of Phaseolus vulgaris (common bean). I've used both SDS and CTAB
>procedures, extensive phenol/chloroform extractions and precipitation.
>Phenol extraction seems to help a little but not enough and I'm not too
>excited about doing 400 phenol extractions. Apparently this is not a new
>problem, a graduate student about 5 years ago had the same problem and
>couldn't find a solution.
>    Has anyone had a similar experience or have any ideas as to how to
>solve this problem ??
>
>Thank you for any information you may provide,
>Margarita
>





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