Marker

Ma Sheila M. De Jesus mshmdeje at USTCC.ust.edu.ph
Wed Feb 23 22:58:04 EST 2000


Hi,
Thanks for all those who replied.  I have a follow-up question. We are
optimizing the use of 16SrDNA in our study. We are a little new to this
endeavor, howver, when I run PCR on ATCC E.coli, the gel showed 2 bands:
1st band between 1330-1584 and the second band near 983. The Dna isolation
i used is the genereleaser protocol.  Since I used the universal primers
on this, I expect only the1st band.  Could the second band be a
contaminant?  

Again, any advice is highly appreciated.

Thanks.

Sheila 
 *********************

On Wed, 23 Feb 2000, Dr. Duncan Clark wrote:

> In article <Pine.OSF.3.96.1000223143548.2605A-100000 at USTCC.ust.edu.ph>,
> Ma Sheila M. De Jesus <mshmdeje at USTCC.ust.edu.ph> writes
> >Does anyone know the meaning of the different bands in lambda DNA EcoRI + Hind 
> >III Marker from Promega
> 
> Fragment sizes: 21226, 5148, 4973, 4277, 3530, 2027, 1904, 1584, 1330,
> 983, 831, 564 and 125bp.
> 
> Duncan
> 
> -- 
> The problem with being on the cutting edge is that you occasionally get 
> sliced from time to time....
> 
> Duncan Clark
> DNAmp Ltd.
> Tel: +44(0)1252376288
> FAX: +44(0)8701640382
> http://www.dnamp.com
> http://www.genesys.demon.co.uk
> 

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