how to insure that a protein is nuclear or not?

Denis Dupuy denis.dupuy at
Thu Feb 24 06:49:47 EST 2000

Hi everybody,

My prot display strong NLS but immunocytochemystry failed to detect it
in the nucleus.
How could i cheeck whether it's because it doesn't enter the nucleus  or
because its epitope become inaccessible when in the nucleus?

Thanks for your help.


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