how to insure that a protein is nuclear or not?

[Denis Dupuy]

Hi everybody,

My prot display strong NLS but immunocytochemystry failed to detect it
in the nucleus.
How could i cheeck whether it's because it doesn't enter the nucleus  or
because its epitope become inaccessible when in the nucleus?

Thanks for your help.

--



DUPUY Denis
Laboratoire de Pathologie Moléculaire
et Thérapie Génique
Universite V. Segalen, Bordeaux 2
146 rue Léo Saignat
33076 Bordeaux Cedex

Tel: (33) 557 57 10 10
     Secrétariat 13 73, poste 6476 ou  6503
Fax: (33) 556 98 33 48


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