Glycerol: v/v or w/v?
Wolfgang.Schechinger at med.uni-tuebingen.de
Thu Feb 24 17:58:34 EST 2000
No. Not obviously. I use w/v. Did you ever try to pipet 100%
glycerol? For this reason, one always should how the dilution was
In real life, it normally should not matter. What matters more is,
that there is a difference if you have 50 ml of glycerol and add
water q.s. 100 ml or if you have 50 ml of water and add glycerol q.s.
100 ml. For understanding this phenomenon, mix 50 ml of ethanol
**and** 50 ml of water in a graded cylinder (the experiment is easier
to perform with EtOH).
> From: Colin Rasmussen <colin at schizo.usask.ca>
> Subject: Re: Glycerol: v/v or w/v?
> Date: Thu, 24 Feb 2000 16:20:52 -0600
> Organization: University of Saskatchewan
> To: methods at hgmp.mrc.ac.uk
> John Rebers wrote:
> > When making a solution of glycerol that is supposed to be 50% (for
> > example), is this to be interpreted as a volume/volume percentage
> > (i.e. 50 ml of glycerol in 100 ml final volume) or a weight/volume
> > pecentage (i.e. 50 g or glycerol in 100 ml final volume). I
> > suppose it could also be interpreted as a weight/weight
> > solution.... Since glycerol is a liquid, I'd always used the
> > volume/volume interpretation, but just ran across someone who was
> > using weight/volume instead. Curious as to which was correct, or
> > at least more commonly used.
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Dr. Wolfgang Schechinger, Dept. of Pathobiochemistry
University of Tuebingen, Germany
email: wolfgang.schechinger at med.uni-tuebingen.de
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