RT-PCR Problem///

mqh mqh at mh.bj.col.com.cn
Thu Feb 24 23:08:41 EST 2000


Dear Netters:
I cloned a cDNA by RT-PCR using degenerate primers. The strange thing
is, when I compare the genes with other plants, I find our clone only
contain the exon 1, 2, 3 and 6, but do not contain the exon 4 and 5.
Anyone met this or any seggestion will be very appreciative. Thanks.
Please reply to my
personel addreess.
Dr Q.H.Ma
Institute of Botany, Academia Sinica
Beijing 100093, China


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