RT-PCR Problem///

mqh mqh at mh.bj.col.com.cn
Thu Feb 24 23:08:41 EST 2000

Dear Netters:
I cloned a cDNA by RT-PCR using degenerate primers. The strange thing
is, when I compare the genes with other plants, I find our clone only
contain the exon 1, 2, 3 and 6, but do not contain the exon 4 and 5.
Anyone met this or any seggestion will be very appreciative. Thanks.
Please reply to my
personel addreess.
Dr Q.H.Ma
Institute of Botany, Academia Sinica
Beijing 100093, China


More information about the Methods mailing list