low expression of GFP-fusion

Martin Offterdinger martin.offterdinger at akh-wien.ac.at
Fri Feb 25 05:43:52 EST 2000


Hi!
This is probably a common problem; I would like to stably express a
EGFP-Cterminal fusion protein in a mammary epithelial cell line. The
construct is quite large (final size 215kD). I only get clones with a very
low expression making it difficult to analyze the expression by fluorescence
microscopy. I used Clontechs pEGFP-C1 vector.
What can i do to improve expression/fluorescence.
Are there other vectors that are more suitable??
Should I try a different cell line?
Could I improve the expression on the clone that I already have?
Thanks!
Martin






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