Stable Transfection of Cells in Suspension

Mikkel mikpedNOmiSPAM at
Fri Feb 25 09:01:30 EST 2000

Hi !

I have a problem regarding stable transfection of cells in
suspension. I use the Tet-On system from clontech and my
gene of interest is EGFRvIII. My problem is that every time
i put a selction pressure on the transfected cells the
non-transfected cells die (as they should) but the contents
released from these cells kill the transfected ones.
Does anyone know of an easy way of how to separate the dying
cells from the living ?



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