thermostable reverse transcriptase

Richard J. Dudley rdudley at pitt.edu
Fri Feb 25 08:25:32 EST 2000


There are two options for you.  Tth has RT activity in Mn buffer; check
out Promega's catalog (www.promega.com) for Tth.  We've fiddled with Tth
a little bit, and have out it aside.  The results weren't stellar.

I haven't tried this second option yet, but apparently trehalose will
stabilize MMuLV RT up to 60C.  PNAS 95:520-524.  We've ordered the
trehalose.

You might also want to read J Clin Microbiol 35(3): 570-577

Good luck!

rich

"Frank O. Fackelmayer" wrote:

> Hi all,
> We are looking for a thermostable reverse transcriptase to make cDNA
> from a template with very strong secondary structures. I´m quite sure
> I´ve seen ads for such an enzyme, but I really cannot recall details.
> Any suggestions? suppliers?
>
> Thanks a lot,
> Frank

--
Richard J. Dudley (rdudley+ at pitt.edu)
Research Specialist V
Cystic Fibrosis Research Center
Dept. of Cell Biology and Physiology
University of Pittsburgh
http://www.cbp.pitt.edu
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