thermostable reverse transcriptase

Dr. Duncan Clark Duncan at
Fri Feb 25 08:43:50 EST 2000

In article <38B64252.F765465B at>, Frank O. Fackelmayer
<Frank.Fackelmayer at> writes
>We are looking for a thermostable reverse transcriptase to make cDNA from a 
>template with very strong secondary structures. 

You can use standard AMV RT at 60C but it will die in about 5-10mins.
however if your target is short it would work.

Alternatives include Tth and Roche Diagnostics have another thermophile
that has Mg specific RT activity.

The problem with being on the cutting edge is that you occasionally get 
sliced from time to time....

Duncan Clark
DNAmp Ltd.
Tel: +44(0)1252376288
FAX: +44(0)8701640382

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