thermostable reverse transcriptase

Dr. Duncan Clark Duncan at nospam.demon.co.uk
Fri Feb 25 08:43:50 EST 2000


In article <38B64252.F765465B at uni-konstanz.de>, Frank O. Fackelmayer
<Frank.Fackelmayer at uni-konstanz.de> writes
>We are looking for a thermostable reverse transcriptase to make cDNA from a 
>template with very strong secondary structures. 

You can use standard AMV RT at 60C but it will die in about 5-10mins.
however if your target is short it would work.

Alternatives include Tth and Roche Diagnostics have another thermophile
that has Mg specific RT activity.

Duncan
-- 
The problem with being on the cutting edge is that you occasionally get 
sliced from time to time....

Duncan Clark
DNAmp Ltd.
Tel: +44(0)1252376288
FAX: +44(0)8701640382
http://www.dnamp.com
http://www.genesys.demon.co.uk




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