total RNA for RT-PCR from

The Lab Rat thelabrat at thelabrat.com
Fri Feb 25 11:59:30 EST 2000


dabram wrote in message <38B114A5.970C12CD at biotop.umcs.lublin.pl>...
>Dear all,
>I search for kits yields ultra-pure total RNA from yeast (Candida) for
>better results in RT-PCR. The best where is option of treatment with
>DNase (RNase-free).
>Could anyone send some information about firms which offer such kit.
>I shall appreciate any information.
>Thanks in advance,
>Darek Abramczyk
>dabram at biotop.umcs.lublin.pl
>
Here is a kit from Bio101 for yeast:

http://www.bio101.com/rna-kits/fastrna-red.html

They don't have a DNAse step in the protocol, but if you need it then you
can just add it at the end of the protocol and heat inactivate after digest.

JeffF

-----------------------------------------------------------------------
Jeff Fairman, Ph.D.
Senior Scientist, Pharmacogenomics Research
Clingenix, Inc.
871 Industrial Road, Suite J
San Carlos, CA 94070
(650) 598-7645 (office)
(650) 598-7641 (fax)
jfairman at clingenix.com


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