how to insure that a protein is nuclear or not?

[Thomas Floss]

Hi Denis,
making a fusion with GFP might give you the answer as well.
Best, Thomas

Denis Dupuy wrote:

> Hi everybody,
>
> My prot display strong NLS but immunocytochemystry failed to detect it
> in the nucleus.
> How could i cheeck whether it's because it doesn't enter the nucleus  or
> because its epitope become inaccessible when in the nucleus?
>
> Thanks for your help.
>
> --
>
> DUPUY Denis
> Laboratoire de Pathologie Moléculaire
> et Thérapie Génique
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>
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