how to insure that a protein is nuclear or not?

Thomas Floss tfloss at gsf.de
Sat Feb 26 04:38:48 EST 2000


Hi Denis,
making a fusion with GFP might give you the answer as well.
Best, Thomas

Denis Dupuy wrote:

> Hi everybody,
>
> My prot display strong NLS but immunocytochemystry failed to detect it
> in the nucleus.
> How could i cheeck whether it's because it doesn't enter the nucleus  or
> because its epitope become inaccessible when in the nucleus?
>
> Thanks for your help.
>
> --
>
> DUPUY Denis
> Laboratoire de Pathologie Moléculaire
> et Thérapie Génique
> Universite V. Segalen, Bordeaux 2
> 146 rue Léo Saignat
> 33076 Bordeaux Cedex
>
> Tel: (33) 557 57 10 10
>      Secrétariat 13 73, poste 6476 ou  6503
> Fax: (33) 556 98 33 48
>
> ------------------------------------------------------------------------------------------------------------------------------------------------------------
>
> "Early to rise, early to bed, makes a man healthy, wealthy and dead."
>
>     Terry Pratchett (The Light Fantastic)





More information about the Methods mailing list