SSH and blunt ligation!

Csaba Kiss csakis at ki.se
Sun Feb 27 06:56:02 EST 2000


Hi!
I was wondering why do people use blunt ligation to ligate adaptors to the ends of the cDNA fragments after digestion with RsaI. This enzyme creates blunt ends. If one uses i.e Sau3AI one would have a 4 bp overhang and the ligation would be much easier.
Is there a problem with concatamerization?

Csaba
csakis at ki.se
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