SSH and blunt ligation!

[Csaba Kiss]

Hi!
I was wondering why do people use blunt ligation to ligate adaptors to the ends of the cDNA fragments after digestion with RsaI. This enzyme creates blunt ends. If one uses i.e Sau3AI one would have a 4 bp overhang and the ligation would be much easier.
Is there a problem with concatamerization?

Csaba
csakis at ki.se
-------------- next part --------------
An HTML attachment was scrubbed...
URL: http://iubio.bio.indiana.edu/bionet/mm/methods/attachments/20000227/cf377e6c/attachment.html