excessive bps to NdeI

[Peter]

On 27 Feb 2000 15:30:40 -0000, zhongyi at sunm.shcnc.ac.cn wrote:

I cloned PCR fragments using a NdeI site. From my experice 6 bp are
enough to get sufficient restriction. 
Peter



>Hello
>   if I want to improve the cleavable efficiency of primer with NdeI recognition site,how many excessive bps should be added to side of the NdeI recognition sequence? 
>The cleavage efficiency is 0%  when only 1 base is added .Can anybody give me more information ?
>   Thanks in advance
>
>   
>
>            Biochemistry
>            Biochem at kali.com.cnYuan Zhongyi
>
>
>---