excessive bps to NdeI
a9001527 at unet.univie.ac.at
Mon Feb 28 02:07:32 EST 2000
On 27 Feb 2000 15:30:40 -0000, zhongyi at sunm.shcnc.ac.cn wrote:
I cloned PCR fragments using a NdeI site. From my experice 6 bp are
enough to get sufficient restriction.
> if I want to improve the cleavable efficiency of primer with NdeI recognition site,how many excessive bps should be added to side of the NdeI recognition sequence?
>The cleavage efficiency is 0% when only 1 base is added .Can anybody give me more information ?
> Thanks in advance
> Biochem at kali.com.cnYuan Zhongyi
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