excessive bps to NdeI

Biochemistry Biochem at kali.com.cn
Mon Feb 28 04:53:17 EST 2000


Hello
   if I want to improve the cleavable efficiency of primer with NdeI recognition site,how many excessive bps should be added to side of the NdeI recognition sequence? 
The cleavage efficiency is 0%  when only 1 base is added .Can anybody give me more information ?
   Thanks in advance

   

            Biochemistry
            Biochem at kali.com.cn

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