Western - tiny tissue amounts
kempfert at uke.uni-hamburg.de
Mon Feb 28 05:54:18 EST 2000
First thanks for your interest.
The main problem for us is: how to crush those small pieces of tissue (here talking
about 20mg each) and then to get the cytosol.
Would be very kind if you could help us.
> In article <38B664E6.97A9B956 at uke.uni-hamburg.de>, =?iso-8859-1?Q?J=F6rg?=
> Kempfert <kempfert at uke.uni-hamburg.de> wrote:
> > We want to do some western blot analyses with really small tissue
> > amounts. Does anybody have experience with this kind of problem? It
> > would be very nice if someone could help us in this case.
> > Looking forward to get your hints.
> What is the problem? I routinely use very small pieces of tissue for westerns.
> Peter Pediaditakis
> University of Pittsburgh
> Dept. of Pathology
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