Western - tiny tissue amounts

Jörg Kempfert kempfert at uke.uni-hamburg.de
Mon Feb 28 05:54:18 EST 2000


First thanks for your interest.

The main problem for us is: how to crush those small pieces of tissue (here talking
about 20mg each) and then to get the cytosol.

Would be very kind if you could help us.

Joerg Kempfert


pathos schrieb:

> In article <38B664E6.97A9B956 at uke.uni-hamburg.de>, =?iso-8859-1?Q?J=F6rg?=
> Kempfert <kempfert at uke.uni-hamburg.de> wrote:
>
> > We want to do some western blot analyses with really small tissue
> > amounts. Does anybody have experience with this kind of problem? It
> > would be very nice if someone could help us in this case.
> >
> > Looking forward to get your hints.
>
> What is the problem?  I routinely use very small pieces of tissue for westerns.
>
> --
> Peter Pediaditakis
> University of Pittsburgh
> Dept. of Pathology





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