excessive bps to NdeI

Dr. Duncan Clark Duncan at nospam.demon.co.uk
Mon Feb 28 06:50:40 EST 2000


In article <38ba1e26.3638928 at news.univie.ac.at>, Peter
<a9001527 at unet.univie.ac.at> writes
>I cloned PCR fragments using a NdeI site. From my experice 6 bp are 
>enough to get sufficient restriction. 

We use 6 extra bp all the time here. It is one of our main RE's (NEB)
used for cloning at the ATG in various expression vectors.

Duncan
-- 
The problem with being on the cutting edge is that you occasionally get 
sliced from time to time....

Duncan Clark
DNAmp Ltd.
Tel: +44(0)1252376288
FAX: +44(0)8701640382
http://www.dnamp.com
http://www.genesys.demon.co.uk




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