excessive bps to NdeI
Dr. Duncan Clark
Duncan at nospam.demon.co.uk
Mon Feb 28 06:50:40 EST 2000
In article <38ba1e26.3638928 at news.univie.ac.at>, Peter
<a9001527 at unet.univie.ac.at> writes
>I cloned PCR fragments using a NdeI site. From my experice 6 bp are
>enough to get sufficient restriction.
We use 6 extra bp all the time here. It is one of our main RE's (NEB)
used for cloning at the ATG in various expression vectors.
The problem with being on the cutting edge is that you occasionally get
sliced from time to time....
More information about the Methods