Taqman Assay Question
mail at mail.gov
Mon Feb 28 09:14:29 EST 2000
We have recently obtained a 7700 SDS machine from PE.
We want to do allelic discrimination assays.
My question is does anyone have a general protocol for the set up of the
PCR master mix and thermal cycler conditions?
Such as the amounts of primers, probes, buffer dNTPs, etc.
We would like to stay away from the Universal Master mix they are
pushing, but they are not offering any technical support for assays that
do not use their Universal Master mix.
This is sad, considering the amount we have already paid for the unit.
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