PCR problems using Streptomyces cosmid DNA as template?

Colin Dolphin colin.dolphin at kcl.ac.uk
Mon Feb 28 09:21:39 EST 2000


Dear All


I'm attempting to amplify a predicted gene in Streptomyces coelicolor
using the appropriate cosmid (approx. 30k) DNA, containing the gene
sequence, as template without, so far, any success. I was wondering
whether this could be due to the high GC content of the template. Would
fragmenting the cosmid with an appropriate RE help and/or the use of an
additive such as DMSO? 


Colin Dolphin
Dept of Pharmacy
Kings College London
London SE1 8WA
colin.dolphin at kcl.ac.uk




More information about the Methods mailing list