Glycon in ligations?

Colin Rasmussen colin at schizo.usask.ca
Mon Feb 28 10:10:12 EST 2000



Wolfgang Schechinger wrote:

> Hi all,
>
> Does anyone have experience with glycogen present in DNA ligations?
>
> I'd like to recover a small quantity of DNA by EtOH precipitation and
> I thought I could increase the yield by adding a small amount of
> glycogen to the precipitation.
> Will it hamper the ligation I intend to use the DNA for?

Not in my experience.  We used to use it all the time for that sort of
thing.

Colin





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