sephadex problem

[Wolfgang Schechinger]

hi all, 

in a protocol, one needs sephadex g25 fine. on my shelf there is g25 
coarse. does this just mean big / small beads or is there a important 
difference i should take notice of? the protocol uses sephadex for 
separating a 80 kd protein from low mw impurities after a chemical 
modification. 
generally, a pointer to gel filtartion theory would be helpful to me.

thanks for all input!

wolfgang
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Dr. Wolfgang Schechinger, Dept. of Pathobiochemistry
University of Tuebingen, Germany
email: wolfgang.schechinger at med.uni-tuebingen.de 
wwWait: http://www.medizin.uni-tuebingen.de/~wgschech/start.htm
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usual disclaimers apply 
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