Western - tiny tissue amounts

Wolfgang Schechinger Wolfgang.Schechinger at med.uni-tuebingen.de
Mon Feb 28 13:29:43 EST 2000


anyway, I'd suggest to homenize your tissue in a buffer containing 1% 
triton x 100 (beneath protease inhibitors and the usual stuff). 
probably a potter device or like is the best for this purpose; there 
are nice tiny pestils (sp?) fitting in eppis, so you need not to be 
afraid that proteins sticking to the tubes.
afterwards, spin down the nuclei and cytoskeleton at high speed.
using an ultraturrax or ultrasonic device probably will mix up the 
nuclei, too. 

wolfgang
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Dr. Wolfgang Schechinger, Dept. of Pathobiochemistry
University of Tuebingen, Germany
email: wolfgang.schechinger at med.uni-tuebingen.de 
wwWait: http://www.medizin.uni-tuebingen.de/~wgschech/start.htm
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