sephadex problem

Nick Theodorakis nicholas_theodorakis at urmc.rochester.edu
Mon Feb 28 15:27:46 EST 2000


In article
<200002281823.TAA21224 at intwww.zit.med.uni-tuebingen.de>
, Wolfgang.Schechinger at med.uni-tuebingen.de ("Wolfgang
Schechinger") wrote:
>hi all,
>
>in a protocol, one needs sephadex g25 fine. on my shelf there
is g25
>coarse. does this just mean big / small beads or is there a
important
>difference i should take notice of? the protocol uses sephadex
for
>separating a 80 kd protein from low mw impurities after a
chemical
>modification.
>generally, a pointer to gel filtartion theory would be helpful
to me.
>
>thanks for all input!
>
>wolfgang

"Fine" has a higher resolution than "coarse" which may not matter
if you are just desalting. Also, "coarse" has a higher flow rate.
A chromatography company -- was it Pharmacia? -- used to publish
a
nice booklet about the theory and practice of gel filtration.


Nick Theodorakis

nicholas_theodorakis at urmc.rochester.edu

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