undigestable genomic DNA

Alexander skorokha at mail.uni-mainz.de
Tue Feb 29 04:21:52 EST 2000


Hi Southern-newbie, :-))

Your genomic DNA could still has DNA-binding proteins. In my case it was
crucial. Sau3A I was able to digest, 6-cutter - not.  I was working with
sponge.

Alexander.

Gregor Bucher schrieb:
> 
> Hi southern-freaks!
> 
> I want to do a southern with genomic DNA (insect). When I digest the DNA
> with Hind3 it looks fine. With EcoR1 and Xho however, there is quiete a
> bit high molecular DNA that obviously has not been digested (digesting 2
> hours or o/n does not change things). Does anybody have experience with
> genomic southerns?
> 
> Should I proceed or start with a new preparation of DNA?
> 
> Thanks for comments!
> 
> Gregor
> --
> Gregor Bucher, Zoologisches Institut der LMU München
> 0049/89/5902/444
> st2153 at zi.biologie.uni-muenchen.de
> ----------------------------------




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