kinetics of cycloheximide

Denis Dupuy denis.dupuy at
Tue Feb 29 05:34:54 EST 2000

Dear helpfull friends,

I want to use CHX on 3T3 transfected cells to check a nuclear
accumulation hypothesis of a GFP fusion protein.
Litterature gave me a range of CHX concentration between 10µg/ml to
200µg/ml and no time consideration...
what's the best concentration of CHX to use and how long does it take to
observe a nuclear accumulation.



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