low copy plasmid isolation

Dr. Duncan Clark Duncan at nospam.demon.co.uk
Tue Feb 29 06:10:16 EST 2000


In article <89ep5p$k5k$1 at newsflash.concordia.ca>, ELAINE B S NEWMAN
<neweb at alcor.concordia.ca> writes
>Can anyone tell me how to get enough plasmid from a cell containing a psc101 
>plasmid in order to cut it and check plasmid structure? 

No easy way other than growing up lots and lots. I don't think the
replicon in pSC101 is amplifiable. Anyone know for sure?

Duncan
-- 
The problem with being on the cutting edge is that you occasionally get 
sliced from time to time....

Duncan Clark
DNAmp Ltd.
Tel: +44(0)1252376288
FAX: +44(0)8701640382
http://www.dnamp.com
http://www.genesys.demon.co.uk




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