low copy plasmid isolation

Dr. Duncan Clark Duncan at nospam.demon.co.uk
Tue Feb 29 06:10:16 EST 2000

In article <89ep5p$k5k$1 at newsflash.concordia.ca>, ELAINE B S NEWMAN
<neweb at alcor.concordia.ca> writes
>Can anyone tell me how to get enough plasmid from a cell containing a psc101 
>plasmid in order to cut it and check plasmid structure? 

No easy way other than growing up lots and lots. I don't think the
replicon in pSC101 is amplifiable. Anyone know for sure?

The problem with being on the cutting edge is that you occasionally get 
sliced from time to time....

Duncan Clark
DNAmp Ltd.
Tel: +44(0)1252376288
FAX: +44(0)8701640382

More information about the Methods mailing list