western blot: colorless protein bands

ITABAJARA DA SILVA VAZ JUNIOR ita at dna.cbiot.ufrgs.br
Tue Feb 29 15:39:11 EST 2000


Dear all, 
I am working with Western-blot.
Hello there, 
In a Western blot the nitrocellulose was blocked with 5% blotto 
and an anti-mouse IgG-phosphatase alkaline conjugate was 
used.
These produce a negative image of colorless protein bands 
against a clear background.
Any help will be appreciated.
__________________________________________________
Itabajara da Silva Vaz Junior
Faculdade de Veterinaria e 
Centro de Biotecnologia do RS
Universidade Federal do Rio Grande do Sul
Av. Bento Goncalves 9500   C.P. 15005
Predio 43421 - Campus do Vale
91501-970  Porto Alegre RS   Brasil
Fone (51) 3166078    Fax (51)319-10-79  [00 55 51 3191079]
E-mail: ITA at DNA.CBIOT.UFRGS.BR  
http://www.ufrgs.br/depbiot/201/cbt201.htm      
http://www.ufrgs.br/favet/imunovet
---




More information about the Methods mailing list