Basic e'poration advice
clarosa at biocomp.unl.edu
Tue Feb 29 18:39:22 EST 2000
John Dixon wrote:
> Hi, I used to make e'comp cells a while back and have just made several
> new batches by the same method (the GYT resuspension protocol that was
> published in TIBS technical tips a few years ago).
> We have a new GenePulserII now and although the competency of my cells
> is OK (1-5x10E8), the time constants when I use my cells are 2-2.5
You may have a little more solutes left over in cells than before... What
is the problem? if they dont arc, and the competency is ok , I would not
worry any more about it. Try reducing the volume of cells by halve.
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