sephadex problem

Chris LaRosa clarosa at biocomp.unl.edu
Tue Feb 29 18:42:24 EST 2000



Wolfgang Schechinger wrote:

> hi all,
>
> in a protocol, one needs sephadex g25 fine. on my shelf there is g25
> coarse. does this just mean big / small beads or is there a important
> difference i should take notice of? the protocol uses sephadex for
> separating a 80 kd protein from low mw impurities after a chemical
> modification.

The course is probably ok for larger chromatographic columns or batch
purifications.  The Course means larger beads.   For mini spin columns
finer is probably better.





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