Methods February 2000 Archives by author

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Starting: Tue Feb 1 04:42:49 EST 2000
Ending: Tue Feb 29 23:22:23 EST 2000
Messages: 816

• how to do a membrane protein prep from Xenopus?   -7758,599-3231
• can I make my own poly dT primer   =?gb2312?q?sam=20wayne?=
• RNA extraction from e. coli for northern   Frederik Börnke
• Trivial transformation improvement   Frederik Börnke
• Trivial transformation improvement   Frederik Börnke
• Trivial transformation improvement   Frederik Börnke
• where do you purchase your restriction enzymes?   Frederik Börnke
• where do you purchase your restriction enzymes?   Frederik Börnke
• amino-triazole   Frederik Börnke
• What determines the qualitity of an antibody?   Frederik Börnke
• thermostable reverse transcriptase   Frederik Börnke
• Kit for RNA-isolation?   Dr. Dietrich Mäde
• LexA compatible with Gal4   Tim Söllick
• LexA compatible with Gal4   Tim Söllick
• LexA compatible with Gal4   Tim Söllick
• a yeast two-hybrid system with C-terminal fusion proteins?   Tim Söllick
• RNA problem   Francisco Alba =?x-user-defined?Q?Arag=FCez?=
• RACE and Clonetech   Karthik Aghoram
• RNA from <100 mg leaf tissue   Karthik Aghoram
• "conservative" mutagenesis withinin SV40 promotor   Ashok Aiyar
• undigestable genomic DNA   Alexander
• pBR322 primers   "David Alexander
• RNase inhibitors   Byran Alexander
• Tobacco acid pyrophosphatase source?   Alexandre.Blais at crchul.ulaval.ca
• CODEHOP PCR   Aniko
• Quantitative PCR   Lluís Armengol
• 15 selection marker   Lluis Armengol
• Sequence analysis programs   Lluis Armengol
• RACE and Clonetech   Peter Ashby
• pBR322 primers   Joseph C. Bagshaw
• Primer Problem   Joseph C. Bagshaw
• Glyceraldehyde-3-phosphate source?   Tim Barnett
• Tissue and DNA extraction from paraffin blocks   Britta Bauche
• Nothern with 290bp probe   Beat.Reidy at hgmp.mrc.ac.uk
• where to get a cDNA clone of bovine pancreatic ribonuclease (RNase-S)   Craig Belcher
• Kit for RNA-isolation?   Belscotia
• Reference for "DNA is an organisms way of making more DNA"   Nico van Belzen
• Gel drying pump   Michael Benedik
• RNA extraction from soils   Gina Berardesco
• Transfer of proteins and transfer buffer formulations...   Bernard Murray, PhD
• RNA extraction from soils   Bernard Murray, PhD
• laci antibodies   Bernard Murray, PhD
• western blot: colorless protein bands   Bernard Murray, PhD
• PCR optimisation   Yves Bertheau
• Variability of quantitative PCR   Yves Bertheau
• Commercialy pure dsDNA (PCR quality)   Yves Bertheau
• Assessing PCVR inihibitors in a ABI 7700 SDS   Yves Bertheau
• EST databases   Bill_A_Nussbaumer at ms.bd.com
• excessive bps to NdeI   Biochemistry
• Reference for "DNA is an organisms way of making more DNA"   Rory O'Brien. Biotechnology
• hMLH1 regulating exon amplification   TIMAR Botond
• Amicon (Millipore) Electroelutoer   Bill Bowers
• Archiving of plasmid DNA   Chris Boyd
• DNA sequencing apparatus   Dr P. Brennan
• Glycosylated Peptides.   Rick Bright
• Protocol for Mouse IL-4 Elispot   Rick Bright
• Trivial transformation improvement   Rick Bright
• Trivial transformation improvement   Rick Bright
• Trivial transformation improvement   Rick Bright
• RACE and Clonetech   Gregor Bucher
• ho to make a probe??   Gregor Bucher
• undigestable genomic DNA   Gregor Bucher
• sequence with no startcodon   Gregor Bucher
• Bailey's Irish Creme   Gregor Bucher
• Nothern with 290bp probe   Gregor Bucher
• Enzymatic vitelline layer degradation?   Gregor Bucher
• Quantitation methods   Bucky
• BSA in PCR   R. Burkert
• DNA degredation during PCR?   R. Burkert
• RNA extraction from specimens preserved in 100 EtOH?   Thorsten Burmester
• (in Spanich) Jornadas SEBBM-empresa y postdocs   Manuel G. CLAROS
• GCB 2000   CSC
• sequence with no startcodon   CSC
• Trivial transformation improvement   CSC
• excessive bps to NdeI   CSC
• biotylilated oligo non soluble   Carmelo
• Contamination with Nucleic Acids   Carmelo
• cDNA synthesis and linear amplification!   Carrie
• where do you purchase your restriction enzymes?   Francesca Catalano
• pUNI: heard of?   Bruno Cenni
• Promoter activity   Bruno Cenni
• Replication assay   Zhonglin Chai
• Reprobing Dig Northerns   Mike Challen
• 2 ways to improve it   Benedict Chambers
• growth pattern   T V Naga Rama Chander
• looking for a fluorometer/luminometer   Alex Chang
• Measure pH of micro bio samples   R. Chang
• Bioinformatics course in Dublin   Wangxue Chen
• on- and off-rate of antibodies   ChenHA
• on- and off-rate of antibodies   ChenHA
• on- and off-rate of antibodies   ChenHA
• Medium without phosphate   ChenHA
• Medium without phosphate   ChenHA
• Medium without phosphate   ChenHA
• on- and off-rate of antibodies   ChenHA
• oligo cloning   ChenHA
• BL21(DE3) and trc promoter   ChenHA
• BL21(DE3) and trc promoter   ChenHA
• Trivial transformation improvement   ChenHA
• Best bacteria for mammalian protein expression   ChenHA
• cloning the wrong way?   ChenHA
• Trivial transformation improvement   ChenHA
• Trivial transformation improvement   ChenHA
• T4 RNA Ligase   ChenHA
• C-terminal His tag   ChenHA
• Luciferase variation among replicates   T Cheng
• RNA Denaturation on PAGE   Choona
• on-line (distance education) courses?   Stacy Ciufo
• using cheap digital camera to photograph gels   Dr. Duncan Clark
• Taq patent   Dr. Duncan Clark
• 35S-labelled oligo purification   Dr. Duncan Clark
• Building the $100 Thermocycler   Dr. Duncan Clark
• 35S-labelled oligo purification   Dr. Duncan Clark
• Building the $100 Thermocycler   Dr. Duncan Clark
• Building the $100 Thermocycler   Dr. Duncan Clark
• 35S-labelled oligo purification   Dr. Duncan Clark
• Building the $100 Thermocycler   Dr. Duncan Clark
• oligoT sequence!   Dr. Duncan Clark
• 35S-labelled oligo purification   Dr. Duncan Clark
• building the $100 thermocycler   Dr. Duncan Clark
• cDNA synthesis and linear amplification!   Dr. Duncan Clark
• cDNA synthesis and linear amplification!   Dr. Duncan Clark
• How to reduce the viscosity of Nuclear extractions??   Dr. Duncan Clark
• cDNA synthesis and linear amplification!   Dr. Duncan Clark
• oligo cloning   Dr. Duncan Clark
• How to reduce the viscosity of Nuclear extractions??   Dr. Duncan Clark
• building the $100 thermocycler   Dr. Duncan Clark
• 'real-time PCR': which machine to buy??   Dr. Duncan Clark
• can I make my own poly dT primer   Dr. Duncan Clark
• BL21(DE3) and trc promoter   Dr. Duncan Clark
• RNA extraction from e. coli for northern   Dr. Duncan Clark
• can I make my own poly dT primer   Dr. Duncan Clark
• BL21(DE3) and trc promoter   Dr. Duncan Clark
• BL21(DE3) and trc promoter   Dr. Duncan Clark
• Trivial transformation improvement   Dr. Duncan Clark
• Autoclaving glycerol for e'competent cells and other ?s   Dr. Duncan Clark
• Source for plasmid expressing tagged Protein A?   Dr. Duncan Clark
• cloning the wrong way?   Dr. Duncan Clark
• Gradient Maker/Formula Data   Dr. Duncan Clark
• Amplitaq Gold in RapidCycler!   Dr. Duncan Clark
• 96 well plate auto-dispensers   Dr. Duncan Clark
• What gel & conditions to use ?   Dr. Duncan Clark
• Codon bias adjusted E. coli?   Dr. Duncan Clark
• E.coli ori compability   Dr. Duncan Clark
• Marker   Dr. Duncan Clark
• Marker   Dr. Duncan Clark
• T4 RNA Ligase   Dr. Duncan Clark
• thermostable reverse transcriptase   Dr. Duncan Clark
• Mastermixes for capillary PCR   Dr. Duncan Clark
• excessive bps to NdeI   Dr. Duncan Clark
• Basic e'poration advice   Dr. Duncan Clark
• low copy plasmid isolation   Dr. Duncan Clark
• Mastermixes for capillary PCR   Dr. Duncan Clark
• ssDNA   Dr. Duncan Clark
• Get paid to review biological products   William Coetzee
• RACE and Clonetech   Steve Cohen
• Codon bias adjusted E. coli?   Scott J. Coutts
• RACE and Clonetech   Bryan Crawford
• DTA vector   Jill Crittenden
• Codon bias adjusted E. coli?   Paul Cullen
• Primer Problem   Paul Cullen
• Review products- earn cash   Colin Cunningham
• Oligo Source   DOCTORHIM at aol.com
• electroporation   David L. Haviland, PhD
• Stable Transfection of Cells in Suspension   David L. Haviland, PhD
• Your Invited!   Dawn
• EGFP antibody Western   Frederik De
• (NONE)   Marty Dekkers
• PCR and pH of buffer   Mark Denhart
• sequence with no startcodon   Dr. Inge Depoortere
• cDNA synthesis and linear amplification!   John Dixon
• Autoclaving glycerol for e'competent cells and other ?s   John Dixon
• where do you purchase your restriction enzymes?   John Dixon
• Basic e'poration advice   John Dixon
• PCR problems using Streptomyces cosmid DNA as template?   Colin Dolphin
• Need help with immunogold labelling   DonHa
• No Cost   Donald
• No Cost   Donald
• where do you purchase your restriction enzymes?   Craig Draper
• Enzymatic vitelline layer degradation?   Kasper Dreyer
• HA tag sequence.   Richard J. Dudley
• Kozak consensus question   Richard J. Dudley
• RACE and Clonetech   Richard J. Dudley
• how to do a membrane protein prep from Xenopus?   Richard J. Dudley
• Enzymatic vitelline layer degradation?   Richard J. Dudley
• thermostable reverse transcriptase   Richard J. Dudley
• any computationnal method using molecular clock?   Denis Dupuy
• how to insure that a protein is nuclear or not?   Denis Dupuy
• kinetics of cycloheximide   Denis Dupuy
• dissolving biotinylated oligo   ET
• DNA contamination in RNA preparation   Christian Ebeling
• Archiving of plasmid DNA   David M. Eisenmann
• Band distorsion and RFLP   Errol
• transfection of oligos   Scott Estes
• DNA Compression.   Marie Estock
• pCRII Topo sequences, pUC119 doesn't: why?   Biopolymer Facility
• How to reduce the viscosity of Nuclear extractions??   Frank O. Fackelmayer
• oligo cloning   Frank O. Fackelmayer
• cDNA synthesis and linear amplification!   Frank O. Fackelmayer
• PCR and pH of buffer   Frank O. Fackelmayer
• dissolving biotinylated oligo   Frank O. Fackelmayer
• oligo cloning   Frank O. Fackelmayer
• Chemiluminescent detection   Frank O. Fackelmayer
• oligo cloning   Frank O. Fackelmayer
• Help! my colony blots are lighting up like a christmas tree   Frank O. Fackelmayer
• cloning the wrong way?   Frank O. Fackelmayer
• RNase inhibitors   Frank O. Fackelmayer
• Contamination with Nucleic Acids   Frank O. Fackelmayer
• HP-1 antibodies?   Frank O. Fackelmayer
• RNase without RNase   Frank O. Fackelmayer
• EGFP antibody Western   Frank O. Fackelmayer
• GMS-cometition/probe quantitation   Frank O. Fackelmayer
• Questions about DNase I FootPrinting   Frank O. Fackelmayer
• Trivial transformation improvement   Frank O. Fackelmayer
• His6-tagged protein purification   Frank O. Fackelmayer
• how to insure that a protein is nuclear or not?   Frank O. Fackelmayer
• thermostable reverse transcriptase   Frank O. Fackelmayer
• Glycerol: v/v or w/v?   Frank O. Fackelmayer
• Stable Transfection of Cells in Suspension   Frank O. Fackelmayer
• Trivial transformation improvement   David Faguy
• PCR problems using Streptomyces cosmid DNA as template?   M.D. Farrar
• PCR problems using Streptomyces cosmid DNA as template?   Chris Fields
• on- and off-rate of antibodies   Esbjorn Fiers
• where do you purchase your restriction enzymes?   Esbjorn Fiers
• HA tag sequence.   S. Findley
• Building the $100 Thermocycler   Tim Fitzmaurice
• Alternatives to Dextran Sulfate in ISH   George Flentke
• how to insure that a protein is nuclear or not?   Thomas Floss
• quantitizing DNA from gel-electrophoresis   Jered Floyd
• oligo cloning   Jered J Floyd
• oligo cloning   Jered J Floyd
• Unstable insert   Jered J Floyd
• another question on DNA-quatitizing   Jered J Floyd
• where do you purchase your restriction enzymes?   Jered J Floyd
• where do you purchase your restriction enzymes?   Jered J Floyd
• Peptides for T-cell proliferation assay: Advice needed   Mr. P.J. Foley
• T Cell Proliferation- Any experts?   Mr. P.J. Foley
• Recombinant Lysozym   Frank.Moersberger at merck.de
• Archiving of plasmid DNA   Neil Fraser
• FACS analysis   Simon James Frost
• on- and off-rate of antibodies   Will Fuller
• ief gels: samples jumping lanes?   Will Fuller
• N-acetyl glucosamine and ief gels   Will Fuller
• 32P gel developping   Paola Furla
• cancel <38ABFFD3.C8F86B08@physiol.ox.ac.uk>   Paola Furla
• How to reduce the viscosity of Nuclear extractions??   G. Dellaire, Ph.D.
• Recommendations for methods to Concentrate Antibodies   G. Dellaire, Ph.D.
• 49th Meeting of American Society of Human Genetics   G. Dellaire, Ph.D.
• Primer Problem   Warren Gallin
• undigestable genomic DNA   Warren Gallin
• Help! My HisTag Protein won't come off Ni Resin   Gareth
• No subject was specified.   Gareth
• Best bacteria for mammalian protein expression   Gareth
• Mycoplasm help   Gary
• Antibodies for the transcription factors PDX1 and PAX4   Benoit Gauthier
• BL21(DE3) and trc promoter   Dr. Peter Gegenheimer
• Glycon in ligations?   Dr. Peter Gegenheimer
• BL21 for 35-S-Met labeling?   Yoram Gerchman
• Taq patent   Richard P. Grant
• Building the $100 Thermocycler   Richard P. Grant
• Building the $100 Thermocycler   Richard P. Grant
• N-terminal seq/ MS reqd, UK   Richard P. Grant
• quantitizing DNA from gel-electrophoresis   Richard P. Grant
• another question on DNA-quatitizing   Richard P. Grant
• where do you purchase your restriction enzymes?   Richard P. Grant
• cloning the wrong way?   Richard P. Grant
• where do you purchase your restriction enzymes?   Richard P. Grant
• Bailey's Irish Creme   Richard P. Grant
• cloning the wrong way?   Richard P. Grant
• DNA sequencing apparatus   Richard P. Grant
• DNA sequencing apparatus   Richard P. Grant
• Kit for RNA-isolation?   Richard P. Grant
• Immobilisation of urease enzyme on PMMA (acrylic polymer)   Andy Greenhalgh
• Flag column   Gregory P. Adams, Ph.D.
• RNase decontaminating soultion.   Kajetan H. Groicher
• Antibody to advanced glycation end-product?   Jinn-Yuh Guh
• Antibody to advanced glycation end-product?   Jinn-Yuh Guh
• EST databases   Dr Antony Halsall
• EST databases   Dr Antony Halsall
• EST databases   Dr Antony Halsall
• CODEHOP PCR   Andrea Hansen
• CODEHOP PCR   Andrea Hansen
• on-line (distance education) courses?   Andrea Hansen
• Sequence analysis programs   Andrea Hansen
• ief gels: samples jumping lanes?   Phil Hardy
• Chicken antibodies   Alistair Harrison
• E. coli expressing taq pol   Ron Hart
• Semi - HTP TCA pptns   Hartley, Jim
• Digital video capture by iMac   Robert Hartley
• 'real-time PCR': which machine to buy??   Robert Hartley
• Quantitative PCR   Robert Hartley
• can I make my own poly dT primer   Robert Hartley
• Kit for RNA-isolation?   Robert Hartley
• Mycoplasma primer sequences   Robert Hartley
• Mastermixes for capillary PCR   Robert Hartley
• DNA degredation during PCR?   Danielle Harvey
• Fix DNA in agarose gels?   Burkhard Hassel
• Is pcr phenotypic method?   Burkhard Hassel
• Genomic DNA (the Maniatis way) for telomere analysis?   Hastings, Dr R.A.
• Building the $100 Thermocycler   David L. Haviland
• electroporation   David L. Haviland
• Photope vs. Genius(DIG)   Steven Hecht
• hybridization oven any good?   Jaco Hendriks
• Fix DNA in agarose gels?   Jaco Hendriks
• 'real-time PCR': which machine to buy??   Jaco Hendriks
• on-line (distance education) courses?   Jaco Hendriks
• genomic DNA from plants?   Jose I. de las Heras
• In vivo Th determination by DTH assay   Antonieta Herrera
• Immunoprecipitation and subsequent phosphotyrosine western   John Hines
• adenine-deficient media for cAMP?   John Hines
• strange cloning results   Martin Hoehne
• pET23d vector   Aki Hoji
• Cloning transgene inertion site   "T. Ian Simpson Holyrood
• Building the $100 Thermocycler   Louis Hom
• Trivial transformation improvement   Louis Hom
• Trivial transformation improvement   Louis Hom
• electroporation   Louis Hom
• 'real-time PCR': which machine to buy??   Guido Hooiveld
• oligo cloning   S Hopkirk
• oligo cloning   S Hopkirk
• oligo cloning   S Hopkirk
• Taq Patent   Eivind Hovig
• E. coli XL1-Red cells   Y. Huang
• Reference for "DNA is an organisms way of making more DNA"   Roland Hubner
• PCR and UV radiation   Jim Hughes
• PCR and UV radiation   Roland Hübner
• Why Roche deserves the disputed Taq patent   Roland Hübner
• western blot: colorless protein bands   ITABAJARA DA SILVA VAZ JUNIOR
• E-Commerce query   JW
• where do you purchase your restriction enzymes?   JW
• Protein Labeling with FlAsH?   Stefan Jakobs
• Unstable insert   Bas Jansen
• ABI 394   Shaban Javed
• Oligo Source   R. Jayakumar
• hybridization oven any good?   R. Jayakumar
• HELP!!! Novozyme 234 where to get now???   R. Jayakumar
• Radiolabel Mol. Wt. Marker   R. Jayakumar
• Fix DNA in agarose gels?   R. Jayakumar
• Help! my colony blots are lighting up like a christmas tree   R. Jayakumar
• Help! my colony blots are lighting up like a christmas tree   R. Jayakumar
• Help! my colony blots are lighting up like a christmas tree   R. Jayakumar
• Contamination with Nucleic Acids   R. Jayakumar
• Marker   Ma Sheila M. De Jesus
• Marker   Ma Sheila M. De Jesus
• Low RNA yield   Yong Jiang
• test   Yong Jiang
• test   Yong Jiang
• detatch the protein from the resin   Yong Jiang
• P. aeruginosa transformation   Yong Jiang
• want to purify my proten!!!!!!!!   Yong Jiang
• strange cloning results   Yong Jiang
• eluation of small DNA fragments from gel   Johns053 at mc.duke.edu
• excessive bps to NdeI   Johns053 at mc.duke.edu
• Glycon in ligations?   Gys de Jongh
• DNA extraction from diverse samples   Alissa Jourdan
• (NONE)   SURESH KUMAR K.G.
• DNA Compression.   Kadmon
• genomic DNA from plants?   Chris Kafer
• Tobacco acid pyrophosphatase source?   Chris Kafer
• RNA from <100 mg leaf tissue   Chris Kafer
• DNA Gyrase   Jim Kami
• RNase without RNase   Jim Kami
• RNase without RNase   Jim Kami
• SSH and blunt ligation!   Narendra Kaushik
• Western - tiny tissue amounts   Jörg Kempfert
• Western - tiny tissue amounts   Jörg Kempfert
• DTA vector   Heidi Kenis
• Biomek 2000 HDR 'pin' tool   Jason A Kennedy
• T4 RNA Ligase   Marieluise Kirchner
• quantitizing DNA from gel-electrophoresis   Rob Kirkpatrick
• Luciferase variation among replicates   Rob Kirkpatrick
• hairpins in PCR primers   Csaba Kiss
• oligoT sequence!   Csaba Kiss
• cDNA synthesis and linear amplification!   Csaba Kiss
• cDNA synthesis and linear amplification!   Csaba Kiss
• oligo cloning   Csaba Kiss
• mRNA isolation without DNA!   Csaba Kiss
• Amplitaq Gold in RapidCycler!   Csaba Kiss
• SSH and blunt ligation!   Csaba Kiss
• on- and off-rate of antibodies   Dima Klenchin
• electroporation   Dima Klenchin
• freezing protein   Dima Klenchin
• Recombinant Lysozym   Dima Klenchin
• on- and off-rate of antibodies   Dima Klenchin
• transfection of oligos   Dima Klenchin
• transfection of oligos   Dima Klenchin
• Transfer of proteins and transfer buffer formulations...   Dima Klenchin
• Ni affinity columns, preparation, use and recharging of   Dima Klenchin
• ABR, JEM, and advertising   Dima Klenchin
• Trivial transformation improvement   Dima Klenchin
• detection virus methods   Dima Klenchin
• Source for plasmid expressing tagged Protein A?   Dima Klenchin
• electroporation   Dima Klenchin
• electroporation   Dima Klenchin
• Source for plasmid expressing tagged Protein A?   Dima Klenchin
• how to insure that a protein is nuclear or not?   Dima Klenchin
• Taq Patent   Klishis, Michelle
• Alternatives to Dextran Sulfate in ISH   Paul Klosen
• Molecular Biology Reagents Franchise Opportunities   Rory Koenen
• phage display   Rory Koenen
• pSP6/T7-19 or AIMV RNA 4 transciption vector   Alain Kohl
• C-terminal His tag   Alain Kohl
• 49th Meeting of American Society of Human Genetics   Andreas Kranz
• Building the $100 Thermocycler   Cornelius Krasel
• Best bacteria for mammalian protein expression   Cornelius Krasel
• where do you purchase your restriction enzymes?   Cornelius Krasel
• EGFP antibody Western   Cornelius Krasel
• dialysis device   Cornelius Krasel
• Recombinant Lysozym   Kresten
• PAGE gels- can you make up to 20% with Tris/Glycine?   Kresten
• Immortilization of fibroblasts   Vivek Kumar
• ECV 304 Are these endothelial cells?   Klaus Kunath
• BL21(DE3) and trc promoter   Petri Kursula
• BL21(DE3) and trc promoter   Petri Kursula
• Bailey's Irish Creme   Petr Kuzmic
• Promoter activity   Theodosios Kyriakou
• Luciferase variation among replicates   Theodosios Kyriakou
• Promoter activity   Theodosios Kyriakou
• Promoter activity   Theodosios Kyriakou
• Promoter activity   Theodosios Kyriakou
• PCR optimisation   Chris LaRosa
• western blot: colorless protein bands   Chris LaRosa
• Basic e'poration advice   Chris LaRosa
• sephadex problem   Chris LaRosa
• excessive bps to NdeI   Chris LaRosa
• Gel drying pump   Chris LaRosa
• Source for plasmid expressing tagged Protein A?   Chris Larosa
• Source for plasmid expressing tagged Protein A?   Chris Larosa
• Get paid to review biological products   David Leggett
• Brdu staining   Nicolas Lemee
• Trivial transformation improvement   Nicolas Lemee
• Trivial transformation improvement   Nicolas Lemee
• pCRII Topo sequences, pUC119 doesn't: why?   Nicolas Lemee
• HELP: Kontron Betamatic II   Boris Lenhard
• Specific staining of ER or Golgi apparatus   Andrew Leung
• excessive bps to NdeI   Andrew Leung
• testing: Do not read this message   Andrew Leung
• Replication assay   mimi Liao
• human pancreatic cDNA needed   Georg Lipps
• Radiolabel Mol. Wt. Marker   Tracy Jo Litzi
• Fix DNA in agarose gels?   Tracy Jo Litzi
• Alternatives to Dextran Sulphate   Livinus.Emebiri at nre.vic.gov.au
• LexA compatible with Gal4   Locas15
• RNA from Drosophila adults   Locas15
• Sequence analysis programs   Muhammad A. Lodhi
• electroporation   R. John Lye
• oligo cloning   R. John Lye
• Taqman Assay Question   MAG
• Taq Patent   Mackay, John-F {~Auckland}
• Help! my colony blots are lighting up like a christmas tree   Malay
• Taq patent   Rafael Maldonado
• Building the $100 Thermocycler   Rafael Maldonado
• hairpins in PCR primers   Rafael Maldonado
• electroporation   Rafael Maldonado
• Medium without phosphate   Rafael Maldonado
• Medium without phosphate   Rafael Maldonado
• Unstable insert   Rafael Maldonado
• a yeast two-hybrid system with C-terminal fusion proteins?   Rafael Maldonado
• Trivial transformation improvement   Rafael Maldonado
• undigestable genomic DNA   Rafael Maldonado
• DNA sequencing apparatus   Rafael Maldonado
• Trivial transformation improvement   Rafael Maldonado
• His6-tagged protein purification   Angela Manning
• Nothern with 290bp probe   JL Martinez
• fluorescent protein assay   J. Martinez-Irujo
• genomic DNA isolation from mouse whole blood (like 500ul)   Erik Matala
• Band distorsion and RFLP   Margarita Mauro
• 'real-time PCR': which machine to buy??   Tom Maynard
• Biowire.com Product Reviews   McKay.Richard
• 35S-labelled oligo purification   Danny McLaughlin
• 35S-labelled oligo purification   Danny McLaughlin
• Low RNA yield   Danny McLaughlin
• 35S-labelled oligo purification   Danny McLaughlin
• Good nuclear extract protocol?   Gary McLean
• Transfer of proteins and transfer buffer formulations...   Neal Robert Melvin
• Immunoprecipitation and subsequent phosphotyrosine western   Neal Robert Melvin
• mRNA isolation into PCR plates   Patrick Merel
• Oligo Source   John Scott Meschke
• Cloning transgene inertion site   David Micklem
• Bailey's Irish Creme   David Micklem
• Stable Transfection of Cells in Suspension   Mikkel
• R: Kozak consensus question   Monod
• fluorescent DNA size standards   Joanita Monteiro
• DOP-PCR whole genome amplification   Joanita Monteiro
• centrifugating colloidal gold   MIguel Moreno
• GAL4 DBD antibody   D.D. Morgan
• pWZLneo   Mr. G. Morley
• HELP!!! Novozyme 234 where to get now???   Dirk Müller
• Genomic phage bank   Dirk Müller
• low copy plasmid isolation   ELAINE B S NEWMAN
• low copy plasmid isolation   ELAINE B S NEWMAN
• Digital video capture by iMac   Tsunehisa Namba
• PCR + KO TARGETING VECTOR   Frauke Naumann
• Fix DNA in agarose gels?   Richard Near
• 35S-labelled oligo purification   News
• 35S-labelled oligo purification   News
• Ad5 transfer vector with IRES   Winston Shim Se Ngie
• hairpins in PCR primers   Michael N. Nitabach
• Check out How to Make Money without Leaving Your House !!!   Norm
• Non-specific bands in PCR   Keith O'Neill
• company willco??   Martin Offterdinger
• EGFP antibody Western   Martin Offterdinger
• low expression of GFP-fusion   Martin Offterdinger
• Oligo Source   Michael Okimoto
• ubiquitin pcr   Elizabeth Osterndorff
• using cheap digital camera to photograph gels   Oswaldo
• Reference for "DNA is an organisms way of making more DNA"   Oswaldo
• Fix DNA in agarose gels?   Ilona Palings
• dialysis device   Dave Parcej
• dna sequencing apparatus   Parent, Eric
• phylogeography analyses info   Parent, Eric
• Glycerol gradients for protein purification   Sean Patterson
• mouse cell culture   Ivan Pavlov
• laci antibodies   Peter
• excessive bps to NdeI   Peter
• Introns in monocot constructs   Joe Petrash
• GAL4 DBD antibody   Christina Philipp
• Where to get RNAse V1   Cathy Phillips
• Gradigels pre-cast PAGE gels. Can anyone reccommend?   Keith Pitts
• PAGE gels- can you make up to 20% with Tris/Glycine?   Keith Pitts
• NEW NEUROSCIENCE INFORMATION CENTER ON THE WEB   PradipG
• intracellular FACS staining - HELP!   Kiley R. Prilliman
• Primer removal   Dr.Narayana R.Isola
• fluorescent protein assay   Jean-Paul RAFFIN
• ssDNA   Jean-Paul RAFFIN
• Fix DNA in agarose gels?   Colin Rasmussen
• Radiolabel Mol. Wt. Marker   Colin Rasmussen
• Radiolabel Mol. Wt. Marker   Colin Rasmussen
• How to reduce the viscosity of Nuclear extractions??   Colin Rasmussen
• oligo cloning   Colin Rasmussen
• Bailey's Irish Creme   Colin Rasmussen
• Bailey's Irish Creme   Colin Rasmussen
• Bailey's Irish Creme   Colin Rasmussen
• Alternatives to Dextran Sulphate   Colin Rasmussen
• Glycerol: v/v or w/v?   Colin Rasmussen
• Glycon in ligations?   Colin Rasmussen
• Variability of quantitative PCR   The Lab Rat
• 'real-time PCR': which machine to buy??   The Lab Rat
• total RNA for RT-PCR from   The Lab Rat
• Taqman Assay Question   The Lab Rat
• Glycerol: v/v or w/v?   John Rebers
• Virus purification   Redflag
• UV-Cross   Kerstin Reisinger
• What gel & conditions to use ?   Jan De Riek
• Unstable insert   Ivo Rieu
• pUNI: heard of?   Ivo Rieu
• RNA from <100 mg leaf tissue   Ivo Rieu
• RNase decontaminating soultion.   Rimas
• Monitor pO2, pH, pCO2 in flowing bio streams   C. Roberts
• Spares for old Zeiss m'scope   Ian Roberts
• Low RNA yield   Karen Roberts
• Low RNA yield   Karen Roberts
• Mycoplasm help   Dr. Fernando Rodriguez-Pascual
• Promoter activity   Dr. Fernando Rodriguez-Pascual
• standard p21/WAF1   Pavel Rossner
• 35S-labelled oligo purification   Hiranya S. Roychowdhury
• Radiolabel Mol. Wt. Marker   Hiranya S. Roychowdhury
• How to reduce the viscosity of Nuclear extractions??   Hiranya S. Roychowdhury
• How to reduce the viscosity of Nuclear extractions??   Hiranya S. Roychowdhury
• oligo cloning   Hiranya S. Roychowdhury
• ho to make a probe??   Hiranya S. Roychowdhury
• oligo cloning   Hiranya S. Roychowdhury
• where do you purchase your restriction enzymes?   Hiranya S. Roychowdhury
• undigestable genomic DNA   Hiranya S. Roychowdhury
• RNase inhibitors   Hiranya S. Roychowdhury
• Help! my colony blots are lighting up like a christmas tree   Hiranya S. Roychowdhury
• Alternatives to Dextran Sulphate   Hiranya S. Roychowdhury
• DNA-conc. in solutions   Hiranya S. Roychowdhury
• Marker   Hiranya S. Roychowdhury
• Glycerol: v/v or w/v?   Hiranya S. Roychowdhury
• sephadex problem   Hiranya S. Roychowdhury
• SV: Primer removal   Dag Rune
• SV: Gel drying pump   Dag Rune
• SV: Glycerol: v/v or w/v?   Dag Rune
• Trivial transformation improvement   George Rutherford
• Pouring transverse urea gradient gels   Amna Saeed
• transfection with COS-1 cells   Dr. N.A. Salazar
• Help! My HisTag Protein won't come off Ni Resin   Neil Saunders
• P. aeruginosa transformation   Neil Saunders
• Building the $100 Thermocycler   Wolfgang Schechinger
• Building the $100 Thermocycler   Wolfgang Schechinger
• Brdu staining   Wolfgang Schechinger
• DC-chol and TC-chol for transfections?   Wolfgang Schechinger
• transfection of oligos   Wolfgang Schechinger
• Ni affinity columns, preparation, use and recharging of   Wolfgang Schechinger
• CODEHOP PCR   Wolfgang Schechinger
• E Coli strain needed   Wolfgang Schechinger
• E. coli XL1-Red cells   Wolfgang Schechinger
• dialysis device   Wolfgang Schechinger
• dialysis device   Wolfgang Schechinger
• Annealing Oligos   Wolfgang Schechinger
• His6-tagged protein purification   Wolfgang Schechinger
• Glycerol: v/v or w/v?   Wolfgang Schechinger
• SV: Glycerol: v/v or w/v?   Wolfgang Schechinger
• Glycon in ligations?   Wolfgang Schechinger
• excessive bps to NdeI   Wolfgang Schechinger
• sephadex problem   Wolfgang Schechinger
• Western - tiny tissue amounts   Wolfgang Schechinger
• Transferrin structure   Wolfgang Schechinger
• "conservative" mutagenesis withinin SV40 promotor   Wolfgang Schechinger
• Transcription Questions   Robert Schoenfeld
• LPS Blotting-Urgent   Holger Scholz
• LPS Transfer   Holger Scholz
• Specific staining of ER or Golgi apparatus   Phillip E. Schwartz
• Annealing Oligos   Andy Scotter
• GST-Fusion problems   Andy Scotter
• FActor Xa Cleavage   Andy Scotter
• amino-triazole   Caron Sebastien
• cloning the wrong way?   Sergio
• undigestable genomic DNA   Sergio
• Genomic phage bank   Sergio
• RNase without RNase   Sergio
• Help with Biosycler oven   Paul Shinn
• Reference for "DNA is an organisms way of making more DNA"   A.F. Simpson
• Building the $100 Thermocycler   A.F. Simpson
• Building the $100 Thermocycler   A.F. Simpson
• Building the $100 Thermocycler   A.F. Simpson
• Looking for a company that sells human hepatocytes   A.F. Simpson
• Promoter activity   A.F. Simpson
• Amplitaq Gold in RapidCycler!   A.F. Simpson
• cDNA synthesis and linear amplification!   Harold E Smith
• fluorescent DNA size standards   Rick Smith
• ABR, JEM, and advertising   Tim Spahlinger
• FDA, patent, e-test   Tim Spahlinger
• Transfection of Jurkat cells?   Jacquelyn Stathopoulos-Connell
• southern hybridizations   Dennis Steinberg
• Bailey's Irish Creme   Kaj Stenberg
• cDNA library kits   Michael L. Sullivan
• oligo cloning   Caroline Szymeczek-Seay
• oligo cloning   Caroline Szymeczek-Seay
• Bailey's Irish Creme   Caroline Szymeczek-Seay
• PCR from blood dots on paper ?   T.Hiltunen
• cultured mouse endothelial cells   Tadashi
• method for transcription start determination   Nick Theodorakis
• the analog of amino acid   Nick Theodorakis
• freezing protein   Nick Theodorakis
• oligo cloning   Nick Theodorakis
• can I make my own poly dT primer   Nick Theodorakis
• mRNA isolation without DNA!   Nick Theodorakis
• ABR, JEM, and advertising   Nick Theodorakis
• ABR, JEM, and advertising   Nick Theodorakis
• Bailey's Irish Creme   Nick Theodorakis
• sephadex problem   Nick Theodorakis
• Flag column   Matt Thomas
• BL21(DE3) and trc promoter   Michael R. Thompson
• Chemiluminescent detection   Tina
• GMS-cometition/probe quantitation   Moez Torki
• Trivial transformation improvement   Moez Torki
• Oligo Source   Jens Tornoe
• cloning the wrong way?   Jens Tornoe
• PCR from blood dots on paper ?   Jens Tornoe
• low expression of GFP-fusion   Jens Tornoe
• Stable Transfection of Cells in Suspension   Jens Tornoe
• Kit for RNA-isolation?   Jesús Torres
• RT-PCR   Jesús Torres
• test   Sigrid Tuble
• test   Sigrid Tuble
• test   Sigrid Tuble
• GST-Fusion problems   Anton Tutter
• Questions about DNase I FootPrinting   Anton Tutter
• BAC Libraries -- How To?????   Shaun Tyler
• the analog of amino acid   Tyson
• Low RNA yield   Tyson
• electroporation   Kevin Urbanic
• Western - tiny tissue amounts   Henk Veldman
• Ni affinity columns, preparation, use and recharging of   Arnoud van Vliet
• Radiolabel Mol. Wt. Marker   Arnoud van Vliet
• Chemiluminescent detection   Arnoud van Vliet
• P. aeruginosa transformation   Arnoud van Vliet
• DNA-conc. in solutions   Arnoud van Vliet
• Marker   Arnoud van Vliet
• Building the Thermocyler. and abi/PElicensing   WSchick at aol.com
• using cheap digital camera to photograph gels   WSchick at aol.com
• Karyotype software   WSchick at aol.com
• Gel drying pump   WSchick at aol.com
• eluation of small DNA fragments from gel   Matthias Wahl
• pSFFV-neo vector?   Bruce Walcheck
• Bailey's Irish Creme   Roxanne Walder
• Oligo Source   Chunxin Wang
• RNA Denaturation on PAGE   Warren
• oligo cloning   Warren
• mRNA isolation without DNA!   Warren
• building the $100 thermocycler   Weining, Song
• building the $100 thermocycler   Weining, Song
• building the $100 thermocycler   Weining, Song
• genomic dna from plants   Weining, Song
• building the $100 thermocycler   Weining, Song
• mastermixes for capillary pcr   Weining, Song
• FDA, patent, e-test   Chong Wai Yin( Zhang Weixian)
• Autoclaving glycerol for e'competent cells and other ?s   Rudi van de Wetering
• Gradient Maker/Formula Data   William A. Rosche, Ph.D.
• subtractive library   Zhencai Wu
• Building the $100 Thermocycler   Jan-Henner Wurmbach
• where do you purchase your restriction enzymes?   Jan-Henner Wurmbach
• where do you purchase your restriction enzymes?   Jan-Henner Wurmbach
• ECV 304 Are these endothelial cells?   Jan-Henner Wurmbach
• Questions about DNase I FootPrinting   XW
• Codon bias adjusted E. coli?   Chun-Song Yang
• Looking for a company that sells human hepatocytes   Yokosawa, Jonny
• ABR, JEM, and advertising   Ian A. York
• ABR, JEM, and advertising   Ian A. York
• genomic DNA from plants?   ZAG
• Non-specific amplification in the control tube   Pablo Zaltz
• BL21 for 35-S-Met labeling?   Miguel Zamora
• Luciferase chromogenic substrate   Mikel Zaratiegui
• HOW to adapt monolayer 293 cells into suspension culture?   Jinsong Zhang
• want to purify my proten!!!!!!!!   Mike Zharkovsky
• Virtualab Web Site Suggestions Needed   Chang Zhu
• Electron microscopy analysis   a.krimer at biosidus.com.ar
• Source for plasmid expressing tagged Protein A?   a.krimer at biosidus.com.ar
• Trivial transformation improvement   a.krimer at biosidus.com.ar
• Glycerol: v/v or w/v?   a.krimer at biosidus.com.ar
• DNA-conc. in solutions   a_firzinger at my-deja.com
• Quantitation methods   a_firzinger at my-deja.com
• cloning the wrong way?   ahmed.elgedaily at dim.usz.ch
• cloning the wrong way?   ahmed.elgedaily at dim.usz.ch
• Using antibiotics when preparing competent cells   ajordaan at my-deja.com
• quantitizing DNA from gel-electrophoresis   aloiv
• another question on DNA-quatitizing   aloiv at my-deja.com
• Fix DNA in agarose gels?   rashie athukorala
• another question on DNA-quatitizing   rashie athukorala
• method for transcription start determination   soenke behrends
• CODEHOP PCR   And'ys bionet
• book,software & product reviewers needed - keep all review titles for your use   carolprice at bigfoot.com
• message quantification   t v naga rama chander
• EVOLUCIÓN BIOLÓGICA WILL BE RENEWED   comunicaciones
• RV: EVOLUCIÓN BIOLÓGICA WILL BE RENEWED   comunicaciones
• Hybridoma Enhancing Supplement   simon cross
• total RNA for RT-PCR from   dabram
• using cheap digital camera to photograph gels   dkim at hector.NMSU.Edu
• Building the $100 Thermocycler   dkim at hector.NMSU.Edu
• Biorad Dcode univeral mutation system   alex dobrovic
• hairpins in PCR primers   alex dobrovic
• Brdu staining   dror_seliktar at my-deja.com
• E.coli ori compability   eforsen at my-deja.com
• size of ribosomal RNA's   karin eizema
• Karyotype software   energy102 at my-deja.com
• electroporation   fairyta
• electroporation   fairyta
• nilanjan sen,IICB,india   ffive
• subscribe   fhaniel
• transfection of oligos   flowman
• FREE NO REPAY CASH GRANTS, UP TOO $50,000!   grantgold at excte.com
• macrophage staining protocol   greet at lmb001.rug.ac.be
• Baculovirus trasnfer vectors for p10 locus?   hdfische at excite.com
• ho to make a probe??   heehalloo at my-deja.com
• Drivers license suspended or revoked? We can help!   idmaster2 at web.com
• annealing oligos   jen_the_glick at my-deja.com
• Phosphorimagers   jens_tornoe at my-deja.com
• hybridization oven any good?   jfcg
• zinc concentration   jhjeon2 at snu.ac.kr
• linear ssDNA migartion in agarose   jqpublic at home.net
• Replication assay   karlster
• Primer Problem   khalil
• question on: how to isolate ssDNA???   bernd kneidinger
• Free Cell Phones   ksimms at aomori.com
• &replyto=000f01bf6c67$255f5820$fd878489@nus.edu.sg&subject=Re: the analog of amino acid   lyric lai
• 'real-time PCR': which machine to buy??   lors
• Glycerol: v/v or w/v?   lors
• Mastermixes for capillary PCR   lors
• detection virus methods   m.foti at biosidus.com.ar
• ML1 cells   marianna_alunni at urmc.rochester.edu
• freezing protein   mb
• freezing protein   mb
• E Coli strain needed   mb
• dialysis device   mb
• fluorescent protein assay   mb
• radioactive detergent   mb
• Guaranteed way to instantly have Excellent Credit!!   mikerobbins_afs at hotmail.com
• E. coli XL1-Red cells   winter moon
• RT-PCR Problem///   mqh
• PCR Mismatch   mqh
• RNA extraction from soils   msho at po-box.mcgill.ca
• thermostable reverse transcriptase   nospam at our.site
• CODEHOP PCR   nvahsen at hot.hgmp.mrc.ac.uk
• Mastermixes for capillary PCR   nvahsen at hot.hgmp.mrc.ac.uk
• L`E.G-A,L C,A`B.L,E T-V D,E-S.C.R,A.M.B.L.E.R........... 5333   otvxiu at 2h7u2gf.com
• Unstable insert   panapat at netscape.net
• Low RNA yield   pathos
• another question on DNA-quatitizing   pathos
• Gel drying pump   pathos
• GEL SCANNING/QUANTITATION   pathos
• Western - tiny tissue amounts   pathos
• Western - tiny tissue amounts   pathos
• FActor Xa Cleavage   pathos
• Kit for RNA-isolation?   612 right
• No subject was specified.   612 right
• No subject was specified.   612 right
• RNA Denaturation on PAGE   rogier
• How to reduce the viscosity of Nuclear extractions??   rogier
• UV-Cross   rogier
• fluorescent DNA size standards   rogier
• sequence with no startcodon   rogier
• Contamination with Nucleic Acids   rogier
• DNA Gyrase   rogier
• Gel scanner   gel scanner
• GEL SCANNING/QUANTITATION   gel scanner
• about kit for horse lymphocyte subsets   seyed_ahmed
• kit for horse lymphocytes subsets   seyed_ahmed
• kit for horse lymphocyte subsets   seyed_ahmed
• Discover Hidden Secrets About Your Neighbors   smartwork at 2bmail.co.uk
• INTERNET SPY!   spy2 at post.com
• intracellular FACS staining - HELP!   tronni at my-deja.com
• 'real-time PCR': which machine to buy??   bradley turner
• thermostable reverse transcriptase->destabilizers?   bradley turner
• Seeking taurocholate source   bradley turner
• ECV 304 Are these endothelial cells?   Hendrik vandenBerg
• GAL4 DBD antibody   vivek
• 10 Million Addresses=$79.00   vx56yvxc at excite.com
• looking for RNA gel-Hepes recipe   warner at removespam.accessgenesis.net
• Help for the design of AS-ODN   xiangli87 at yahoo.com
• DNA binding site   xiang yang
• Your Platinum Free Vacation and Airline Tickets!   yzptc at hotmail.com
• Is pcr phenotypic method?   zack_7 at my-deja.com
• Non-specific amplification in the control tube   zack_7 at my-deja.com
• excessive bps to NdeI   zhongyi at sunm.shcnc.ac.cn

Last message date: Tue Feb 29 23:22:23 EST 2000
Archived on: Sun Mar 6 22:10:55 EST 2005

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