SDS in PCR?

Dr. Duncan Clark Duncan at nospam.demon.co.uk
Tue Jan 4 10:02:28 EST 2000


In article <84sm4a$u4j$1 at clematis.singnet.com.sg>, Chong Wai Yin( Zhang
Weixian) <wychong at singnet.com.sg> writes
>Hi will the addition of SDS to PCR mixture affect the process of PCR (eg.
>inhibit PCR)?

For Taq polymerase

Concn (presume final)        Activity

0                               100%
0.001%                          105%
0.01%                           10%
0.1%                            <0.1%

However addition of 0.5% each Tween20 + NP40 reverses the inhibitory
effect of 0.01% SDS.

The enzyme is also happy in 2M Urea, 10% ethanol, <5% DMF and <10%
formamide.

All from PCR Technology, Principles and Applications for DNA
Amplification, Ed. Henry Erlich, Stockton Press. Table 1, page 20 in the
chapter written by David Gelfand. Of course Promega may dispute this
information. I merely pass it on without too much comment.

Duncan
-- 
The problem with being on the cutting edge is that you occasionally get 
sliced from time to time....

Duncan Clark
DNAmp Ltd.
Tel: +44(0)1252376288
FAX: +44(0)8701640382
http://www.dnamp.com
http://www.genesys.demon.co.uk




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