yijst1 at pitt.edu
Tue Jan 4 12:12:10 EST 2000
if you are blunting a 5' overhanging end, you can use Klenow and if you are
doing on a 3' overhanging end, use T4 DNA Polymerase. see the manual of
these enzymes for detail.
Kevin Urbanic wrote:
> I am trying to blunt-end a piece of plasmid DNA with sticky ends. Mung
> Bean is not working and I would like to try something else.
> Can anybody recommend the appropriate enzyme that will fill in the
> single-stranded ends of a piece of plasmid DNA with sticky ends?
> Kevin Urbanic
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