if you are blunting a 5' overhanging end, you can use Klenow and if you are
doing on a 3' overhanging end, use T4 DNA Polymerase. see the manual of
these enzymes for detail.
ying
Kevin Urbanic wrote:
> I am trying to blunt-end a piece of plasmid DNA with sticky ends. Mung
> Bean is not working and I would like to try something else.
>> Can anybody recommend the appropriate enzyme that will fill in the
> single-stranded ends of a piece of plasmid DNA with sticky ends?
>> Thanks,
> Kevin Urbanic
