sticky-end fill-in

[Hiranya S. Roychowdhury]

At 04:56 PM 1/4/00 GMT, Kevin Urbanic wrote:
>I am trying to blunt-end a piece of plasmid DNA with sticky ends.  Mung 
>Bean is not working and I would like to try something else.
>
>
>Can anybody recommend the appropriate enzyme that will fill in the 
>single-stranded ends of a piece of plasmid DNA with sticky ends?  
>
>Thanks,
>Kevin Urbanic
>
>

Klenow or T7DNA polymerase


Dr. Hiranya Sankar Roychowdhury
GENE LAB/ EPPWS
New Mexico State University
Las Cruces, NM 88003
Ph. (505) 646-5785
hroychow at nmsu.edu

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