At 04:56 PM 1/4/00 GMT, Kevin Urbanic wrote:
>I am trying to blunt-end a piece of plasmid DNA with sticky ends. Mung
>Bean is not working and I would like to try something else.
>>>Can anybody recommend the appropriate enzyme that will fill in the
>single-stranded ends of a piece of plasmid DNA with sticky ends?
Klenow or T7DNA polymerase
Dr. Hiranya Sankar Roychowdhury
GENE LAB/ EPPWS
New Mexico State University
Las Cruces, NM 88003
Ph. (505) 646-5785
hroychow at nmsu.edu