dna isolation from barley seeds ?
ricky_boernke at gmx.net
Wed Jan 5 07:31:05 EST 2000
Frank Maiwald schrieb:
> Hi, everybody,
> I am looking for a good 'home made' protocoll for the isolation of genomic
> DNA from barley seeds. Right now, I am thinking about grinding the seeds and
> phenol extraction / EtOH precipitation but I exspect some problems because
> of the starch content.
> Can anyone offer a working, cheep, nontoxic, qiuck, easy ... i.a.w. perfect
> :) protocoll?
> Thanks in advance,
why don't you just take leaves or seedlings as a starting material and
use a standard
protocol. Starch and stuff shouln't be a problem than.
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