dna isolation from barley seeds ?

Frederik Börnke ricky_boernke at gmx.net
Wed Jan 5 07:31:05 EST 2000



Frank Maiwald schrieb:
> 
> Hi, everybody,
> 
> I am looking for a good 'home made' protocoll for the isolation of genomic
> DNA from barley seeds. Right now, I am thinking about grinding the seeds and
> phenol extraction / EtOH precipitation but I exspect some problems because
> of the starch content.
> Can anyone offer a working, cheep, nontoxic, qiuck, easy ... i.a.w. perfect
> :) protocoll?
> 
> Thanks in advance,
> 
> Frank

Hi Frank,

why don't you just take leaves or seedlings as a starting material and
use a standard
protocol. Starch and stuff shouln't be a problem than.

Cheers
Ricky




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