We have been using Boehringer Mannheim's rapid ligation kit for some time now
and find it to work very well. It cuts a day from most cloning procedures and
gives a very high and reproducible yield of recombinants. Does anyone have a
home-brew procedure that works as well as the kit? It is unlikely that
Boehringer has stumbled onto something very novel but our efforts to replicate
their kit have not worked as well as the proprietary reagents.
Thanks for any suggestions