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Nested primers for human ND5 gene need

carrien carrien at ihug.co.nz
Thu Jan 6 05:30:14 EST 2000


Hi there

I'm working on Human mitochondrial point mutations. I need to sequence whole
ND5 gene (2kb). However, I'm currently encounter some difficulties of
desinging primers inside the gene.

I tried couple of online design softwares, however, each one gave me
different primers.

Is anybody out there got  good working primers inside this gene?


Please help me ...........

Thanks in advance

Carrien








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