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Loading capacity of PAGE

Bruno Cenni cenni at cellbio.unige.ch
Thu Jan 6 12:02:08 EST 2000


Hi all,

I was not able to find it in my books, so I'll try it here:

what is the maximal protein loading capacity of standard SDS-PAGE gels
for which you still get resolved bands?

eg. a 0.75 mm thick "mini"gel (1 lane maybe 5mm wide) with 7-15% vs a
1.5 mm "midi" gel (lanes about 10 mm)

TIA

Bruno






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