Hi all, subject says it really.
I used to regularly make >10E8 competency cells using the Inoue method
with XL1s and XL10s, but now I need DH10Bs and they just don't seem to
like the Inoue method in mine and a colleagues hands (10E6 or less in
four attempts). The glycerols stocks are new and if made competent by
CaCl2 method they work OK (~10E6 to 10E7). Any clues?
John Dixon Lab 44 (1223) 334131
Wellcome/CRC Institute Fax 44 (1223) 334089
United Kingdom CB2 1QR e-m: jpcd100 at mole.bio.cam.ac.uk