Does Inoue method work on DH10Bs?

John Dixon jpcd100 at mole.bio.cam.ac.uk
Thu Jan 6 12:15:19 EST 2000


Hi all, subject says it really. 

I used to regularly make >10E8 competency cells using the Inoue method
with XL1s and XL10s, but now I need DH10Bs and they just don't seem to
like the Inoue method in mine and a colleagues hands (10E6 or less in
four attempts). The glycerols stocks are new and if made competent by
CaCl2 method they work OK (~10E6 to 10E7). Any clues?

Cheers 

JD

-- 
John Dixon                    Lab 44 (1223) 334131
Wellcome/CRC Institute        Fax 44 (1223) 334089
Cambridge University
United Kingdom CB2 1QR       e-m: jpcd100 at mole.bio.cam.ac.uk




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