In article <3874CA90.B23DF124 at cellbio.unige.ch>, cenni at cellbio.unige.ch wrote:
>>I was not able to find it in my books, so I'll try it here:
>>what is the maximal protein loading capacity of standard SDS-PAGE gels
>for which you still get resolved bands?
>>eg. a 0.75 mm thick "mini"gel (1 lane maybe 5mm wide)
About 100 ug resolves more or less OK (but no more than ~5 ug per band!).
Make sure to run stacking part at low current (~15 mA/gel).
> with 7-15% vs a
>1.5 mm "midi" gel (lanes about 10 mm)
These guys have slightly lower resolution but generally the same.
Thus ~ 400 ug.