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Loading capacity of PAGE

Dima Klenchin klenchin at REMOVE_TO_REPLY.facstaff.wisc.edu
Thu Jan 6 22:12:26 EST 2000

In article <3874CA90.B23DF124 at cellbio.unige.ch>, cenni at cellbio.unige.ch wrote:
>Hi all,
>I was not able to find it in my books, so I'll try it here:
>what is the maximal protein loading capacity of standard SDS-PAGE gels
>for which you still get resolved bands?
>eg. a 0.75 mm thick "mini"gel (1 lane maybe 5mm wide)

About 100 ug resolves more or less OK (but no more than ~5 ug per band!). 
Make sure to run stacking part at low current (~15 mA/gel). 

> with 7-15% vs a
>1.5 mm "midi" gel (lanes about 10 mm)

These guys have slightly lower resolution but generally the same. 
Thus ~ 400 ug. 

        - Dima

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