Western Blotting Problem

R. John Lye rjl6n at virginia.edu
Fri Jan 7 07:40:47 EST 2000


Mehdi Alimadadi wrote:
>Could it be the transfer buffer (I think I should dilute that
>more)?  If so please provide me with a suitable transfer buffer 
>ingredients (in terms of the chemicals and the amounts and the pH).

I prefer 10 mM CAPs pH 11.0 plus 10% Methanol for the
transfer buffer.  It works well for me.

John




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