dna isolation from barley seeds ?

Rafael Maldonado rmaldonado at ua.es
Fri Jan 7 06:08:54 EST 2000



Frank Maiwald wrote:
> I am looking for a good 'home made' protocoll for the isolation of genomic
> DNA from barley seeds. Right now, I am thinking about grinding the seeds and
> phenol extraction / EtOH precipitation but I exspect some problems because
> of the starch content.

Why don't use the CTAB method from Current Protocols in Molecular
Biology? I have never used it in seeds, but removing polysaccharides
from bacteria is very good.

E-mail me if you need the protocol. Regards,

-Rafa


-- 
Rafael Maldonado
Divison of Genetics
University of Alicante (Spain)




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