Frank Maiwald wrote:
> I am looking for a good 'home made' protocoll for the isolation of genomic
> DNA from barley seeds. Right now, I am thinking about grinding the seeds and
> phenol extraction / EtOH precipitation but I exspect some problems because
> of the starch content.
Why don't use the CTAB method from Current Protocols in Molecular
Biology? I have never used it in seeds, but removing polysaccharides
from bacteria is very good.
E-mail me if you need the protocol. Regards,
-Rafa
--
Rafael Maldonado
Divison of Genetics
University of Alicante (Spain)
