John Dixon wrote:
> I used to regularly make >10E8 competency cells using the Inoue method
> with XL1s and XL10s, but now I need DH10Bs and they just don't seem to
> like the Inoue method in mine and a colleagues hands (10E6 or less in
> four attempts). The glycerols stocks are new and if made competent by
> CaCl2 method they work OK (~10E6 to 10E7). Any clues?
Inoue protocol is optimized for DH5a. It is expected that different
strains will behave different in a chemical transformation protocol.
Electroporation may be your better choice for high competence, since it
is less strain dependent.
Divison of Genetics
University of Alicante (Spain)