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Does Inoue method work on DH10Bs?

Rafael Maldonado rmaldonado at ua.es
Fri Jan 7 06:11:48 EST 2000



John Dixon wrote:
> I used to regularly make >10E8 competency cells using the Inoue method
> with XL1s and XL10s, but now I need DH10Bs and they just don't seem to
> like the Inoue method in mine and a colleagues hands (10E6 or less in
> four attempts). The glycerols stocks are new and if made competent by
> CaCl2 method they work OK (~10E6 to 10E7). Any clues?

Inoue protocol is optimized for DH5a. It is expected that different
strains will behave different in a chemical transformation protocol.
Electroporation may be your better choice for high competence, since it
is less strain dependent.

-Rafa

-- 
Rafael Maldonado
Divison of Genetics
University of Alicante (Spain)




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