in situ hybridization

Gene Lau genelan at netvigator.com
Sat Jan 8 07:17:53 EST 2000


Hi Zhao,

Well, I guess your probe might be too short. Is it possible to subclone a
larger
insert ? I have tried 700 bp - 1.5 kb probes on Xenopus embryos. The result
was great.

Gene

Zhao Qingsun wrote:

> Hi, all:
>
> I need help about the in situ hybridization. I am doing in situ
> hybridization using DIG-labeled RNA probe in zebrafish.The problem now I
> am meeting is that the non-specific signal of sense probe (control) is
> stronger or as that of the antisense probe. My sense probe is 128 base
> long and the antisense probe is 177 base long. I used LiCl to
> precipitate(purify) the probes and denaturing gel shows that each of
> them is one band. Does someone have some suggestions about it? Thank you
> for your help.
>
> Qinghsun Zhao





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